What question did this study set out to answer?

To identify whether 11β-hydroxysteroid dehydrogenase type 2 is expressed in rat Leydig cells and characterize its role in protecting testosterone production against corticosterone.

March 11, 2005EndocrinologyOpen Access

11β-Hydroxysteroid Dehydrogenase 2 in Rat Leydig Cells: Its Role in Blunting Glucocorticoid Action at Physiological Levels of Substrate

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Authors

RGRen‐Shan GeWenzhou Medical University QDQiang DongXinjiang Medical University ENEn-Mei NiuPopulation Council

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Implication

Experimental study demonstrates 11β-HSD2 expression in rat Leydig cells, indicating a cooperative role with 11β-HSD1 in protecting testosterone synthesis from glucocorticoid inhibition.

Key Points

To identify whether 11β-hydroxysteroid dehydrogenase type 2 is expressed in rat Leydig cells and characterize its role in protecting testosterone production against corticosterone.
Assessed 11β-HSD2 gene and protein expression and subcellular localization in rat Leydig cells using PCR, real-time PCR, immunohistochemistry, Western blotting, and enzyme histochemistry.
Treated Leydig cells with antisense oligonucleotides to selectively inhibit 11β-HSD1 or 11β-HSD2, evaluating oxidoreductase activity and testosterone production under corticosterone exposure.
Real-time PCR revealed a 6-fold enrichment of 11β-HSD2 mRNA in Leydig cells relative to whole testis, though absolute transcript levels were approximately 1,000-fold lower than 11β-HSD1.
Antisense knockdown of 11β-HSD1 decreased reductase activity by 60% and oxidation by 25%, whereas selective 11β-HSD2 knockdown reduced oxidase activity by 50%.
Selective inhibition of either 11β-HSD1 or 11β-HSD2 significantly decreased testosterone biosynthesis in the presence of corticosterone.

Cite This Study

Ge et al. (2005) studied this question.

synapsesocial.com/papers/6a893e76abe412682d92d271 https://doi.org/10.1210/en.2005-0046

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Implication

Experimental study demonstrates 11β-HSD2 expression in rat Leydig cells, indicating a cooperative role with 11β-HSD1 in protecting testosterone synthesis from glucocorticoid inhibition.

11β-Hydroxysteroid Dehydrogenase 2 in Rat Leydig Cells: Its Role in Blunting Glucocorticoid Action at Physiological Levels of Substrate

Discussion

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Implication

Key Points

Cite This Study

11β-Hydroxysteroid Dehydrogenase 2 in Rat Leydig Cells: Its Role in Blunting Glucocorticoid Action at Physiological Levels of Substrate

Discussion

Member takes

Implication

Key Points

Cite This Study

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