Diagnostic value of micro-RNAs for coronary artery disease in Egyptian type 2 diabetic patients

With an increasing global burden of coronary artery disease (CAD), early detection and timely management of risk factors are crucial to reduce morbidity and mortality in such patients. Diabetes mellitus (DM) is considered an independent risk factor for the development of CAD. Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential role of circulating miRNA-149, mi-RNA424 and mi-RNA765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-years old) type 2 diabetes mellitus patients. This study included 120 volunteers of both sexes classified as group I: 30 normal subjects; group II: 30 type 2 diabetic patients (T2D), group III: 30 coronary artery disease patients (CAD) and group IV 30 type 2 diabetic patients with coronary artery disease (T2D&CAD). Plasma mi-RNAs, diabetic biomarkers, lipid profile, inflammatory biomarkers and troponin I were determined. Circulating mi-RNA765 levels were elevated in T2DM with CAD group (7.04 ±0.36), compared with T2DM group (2.01 ±0.27). In contrast, circulating mi-RNA149 and mi-RNA424 levels were decreased in T2DM with CAD group (0.9 ±0.09&0.84±0.08), compared with T2DM group (4.28 ±0.32& 4.52 ±0.31) respectively. These results suggest that circulating mi-RNA149, mi-RNA 424 and mi-RNA765 might be non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged diabetic patients.