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August 22, 2026Discover Plants.Open Access

Integrating qualitative and quantitative traits with multivariate analysis to decipher the diversity structure of barley landraces for sustainable agriculture

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Authors

ASAbhisek shresthaKDKrishna Hari DhakalDGDhurva Prasad Gauchan

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Overview

Agronomic field trial reveals substantial phenotypic and genetic diversity across 75 barley landraces, highlighting valuable germplasm for breeding programs.

Key Points

  • Evaluate the agro-morphological and genetic diversity of barley (Hordeum vulgare L.) landraces to identify superior germplasm for breeding and conservation.
  • Evaluated 75 barley landraces using an Alpha Lattice design with two replications under field conditions at CNRM Bardibas, Madhesh Province, Nepal.
  • Measured qualitative and quantitative agro-morphological traits across vegetative and reproductive growth stages.
  • Conducted analysis of variance (ANOVA), Shannon-Wiener diversity index calculations, principal component analysis (PCA), and both K-means and Ward's hierarchical clustering.
  • ANOVA demonstrated highly significant genotypic differences (p < 0.001) for key traits, including days to flowering (78.5–123 days), days to maturity (115–148 days), sterility (5.6–84.6%), and plot yield (3.0–181.2 g/plot).
  • High phenotypic diversity was recorded with Shannon-Wiener diversity indices reaching 0.994 for grain surface, 0.997 for rachilla hair, and 0.976 for grain crease width.
  • PCA showed that PC1 and PC2 accounted for 54.89% of total variance, while cluster analyses consistently grouped landraces into four distinct clusters and highlighted distinct genotypes NGRC7732 and NGRCO7726.

Cite This Study

shrestha et al. (2026) studied this question.

synapsesocial.com/papers/6a895eeeca7ade938187d248https://doi.org/10.1007/s44372-026-00783-x
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