What does this research mean for the field?

Desialylation of endothelial cells reduces barrier permeability and impairs cell adhesion, and these effects are not fully reversed by partial resialylation. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To evaluate how modifying endothelial sialic acid levels influences cell adhesion, barrier permeability, and the sialylation status of podocalyxin and integrin beta-1.

August 22, 2026Scientific ReportsOpen Access

Modulating the sialic acid content of endothelial cells affects their adhesion and barrier permeability

View Full Paper

Ask AI

Bookmark

To evaluate how modifying endothelial sialic acid levels influences cell adhesion, barrier permeability, and the sialylation status of podocalyxin and integrin beta-1.
Depleted endothelial sialic acid using either enzymatic sialidase treatment or targeted knockout of the key biosynthetic enzyme GNE.
Attempted rescue of sialylation in GNE-knockout cells via supplementation with N-acetylmannosamine (ManNAc) or N-acetylneuraminic acid (Neu5Ac).
Assessed target glycoprotein status (podocalyxin and integrin beta-1) alongside functional measures of cell adhesion and barrier permeability.
Both depletion approaches induced sialic acid deficiency, with GNE-knockout cells exhibiting a more pronounced loss than sialidase-treated cells.
Loss of sialic acid led to decreased barrier permeability and negatively impaired endothelial cell adhesion.
Supplementation with ManNAc or Neu5Ac achieved partial resialylation in GNE-knockout cells but failed to fully rescue baseline adhesion and permeability defects.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

In vitro experiment demonstrates that reducing sialic acid alters barrier permeability and impairs endothelial adhesion, highlighting the role of glycans in vascular integrity.

Cite This Study

Müller et al. (2026) studied this question.

synapsesocial.com/papers/6a895f1cca7ade938187d8be https://doi.org/10.1038/s41598-026-66496-z

View Full Paper

Ask AI

Bookmark

Implication

In vitro experiment demonstrates that reducing sialic acid alters barrier permeability and impairs endothelial adhesion, highlighting the role of glycans in vascular integrity.

Modulating the sialic acid content of endothelial cells affects their adhesion and barrier permeability

Key Points

Discussion

Implication

Cite This Study

Modulating the sialic acid content of endothelial cells affects their adhesion and barrier permeability

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider