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April 1, 1992The Journal of Clinical Endocrinology & Metabolism

Hormonal regulation of the growth and steroidogenic function of human granulosa cells

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Authors

EYEu‐Leong YongYong In UniversityDBDavid T. BairdRoyal College of Obstetricians and GynaecologistsRYR YatesMinistry of Health

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Implication

In vitro study reveals stage-dependent hormonal control of proliferation and steroidogenesis in human granulosa cells, suggesting luteinizing hormone halts cell division to drive hormone synthesis.

Key Points

  • To evaluate stage-dependent interactions between gonadotropins (FSH, LH) and IGF-I on DNA synthesis and steroidogenesis across human granulosa cell development.
  • Obtained human granulosa cells at three developmental stages: immature follicles (late luteal to early follicular phase), mature pre-ovulatory follicles, and granulosa-lutein cells via IVF.
  • Cultured cells for 96 hours in serum-free medium with or without LH, FSH, and IGF-I.
  • Assessed DNA synthesis by measuring [3H]methyl thymidine uptake and evaluated steroidogenesis by assaying culture medium for progesterone, estradiol, and aromatase activity.
  • In immature cells, IGF-I and FSH stimulated thymidine uptake and FSH increased aromatase activity, whereas LH had no effect on growth or progesterone production.
  • In mature cells, LH dose-dependently suppressed basal and IGF-I-stimulated thymidine uptake while markedly stimulating steroid production, generating maximal steroidogenic responses up to 3-fold greater than FSH.
  • In granulosa-lutein cells, LH-mediated suppression of DNA synthesis was even more pronounced, accompanied by the highest observed rates of basal and hormone-stimulated steroidogenesis.

Cite This Study

Yong et al. (1992) studied this question.

synapsesocial.com/papers/6a89652cd89205768aeb2602https://doi.org/10.1210/jcem.74.4.1548349
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