What question did this study set out to answer?

To identify novel protein interactions and domains in stargazin required for synaptic AMPA receptor targeting and clustering.

August 25, 2004Journal of NeuroscienceOpen Access

AMPA Receptor Synaptic Targeting Regulated by Stargazin Interactions with the Golgi-Resident PDZ Protein nPIST

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Authors

ACAdolfo E. CuadraUniversity of Massachusetts Amherst SKSheng‐Han KuoNewYork–Presbyterian Hospital YKYoshimi KawasakiShizuoka Medical Center

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Implication

In vitro study demonstrates that stargazin interacts with Golgi-resident nPIST in hippocampal neurons, indicating a mechanism for AMPA receptor synaptic targeting.

Key Points

To identify novel protein interactions and domains in stargazin required for synaptic AMPA receptor targeting and clustering.
Expressed stargazin mutant constructs in cultured hippocampal neurons to map essential functional domains.
Conducted a yeast two-hybrid screen using stargazin residues 243-283 to discover binding partners.
Characterized nPIST-stargazin interactions via in situ hybridization, immunohistochemistry, coimmunoprecipitation, biochemical fractionation, and neuronal transfections.
Identified a cytoplasmic domain (residues 243-283) in stargazin required for stargazin and AMPAR synaptic clustering.
Demonstrated direct interaction and brain colocalization between stargazin residues 243-283 and the Golgi-resident protein nPIST.
Showed that nPIST overexpression enhances AMPAR synaptic clustering, whereas dominant-negative nPIST expression attenuates it.

Cite This Study

Cuadra et al. (2004) studied this question.

synapsesocial.com/papers/6a896bd98371e1a748c9899e https://doi.org/10.1523/jneurosci.1255-04.2004

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Implication

In vitro study demonstrates that stargazin interacts with Golgi-resident nPIST in hippocampal neurons, indicating a mechanism for AMPA receptor synaptic targeting.

AMPA Receptor Synaptic Targeting Regulated by Stargazin Interactions with the Golgi-Resident PDZ Protein nPIST

Discussion

Member takes

Implication

Key Points

Cite This Study

AMPA Receptor Synaptic Targeting Regulated by Stargazin Interactions with the Golgi-Resident PDZ Protein nPIST

Discussion

Member takes

Implication

Key Points

Cite This Study

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