What is the clinical evidence from this study?

Study design: Other. Population: Rotavirus B (RVB) infection. Intervention: Amino acid sequence variability and predicted antigenicity analysis of RVB VP7. Primary outcome: Identification of genotype-specific antigenic sites and nucleotide substitution rates (dN/dS).

December 3, 2017PathogensOpen Access

Longitudinal Surveillance of Porcine Rotavirus B Strains from the United States and Canada and In Silico Identification of Antigenically Important Sites

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Key result

In silico analysis of RVB VP7 from infected pigs identified 9 completely conserved antigenic sites driven by negative selection, highlighting potential targets for subunit vaccine design.

Population

Rotavirus B (RVB) strains from clinically infected pigs in the United States and Canada

Design

Preclinical

Authors

FSFrances K. ShepherdUniversity of Minnesota MMMichael P. MurtaughWalker (United States)FCFangzhou ChenUniversity of Macau

Discussion

Member takes

Implication

May inform swine RVB vaccine antigen selection; leaves open human translation pending in vivo validation.

Structured PICO

Population

Clinically infected pigs in the United States and Canada evaluated for Rotavirus B viral protein 7 sequence variability and antigenicity.

Exposure

In silico characterization of amino acid sequence variability and prediction of antigenicity of RVB viral protein 7 (VP7)

Outcome

Identification of genotype-specific antigenic sites and conserved antigenic sites

Identified highly conserved, negatively selected antigenic residues on RVB VP7 that may serve as candidate regions for a subunit vaccine design.

Cite This Study

Shepherd et al. (2017) studied Rotavirus B (RVB) infection. Amino acid sequence variability and predicted antigenicity analysis of RVB VP7 was evaluated on Identification of genotype-specific antigenic sites and nucleotide substitution rates (dN/dS). In silico analysis of RVB VP7 from infected pigs identified 9 completely conserved antigenic sites driven by negative selection, highlighting potential targets for subunit vaccine design.

synapsesocial.com/papers/6a896fd1a4df84145ecb0b50 https://doi.org/10.3390/pathogens6040064

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Longitudinal Surveillance of Porcine Rotavirus B Strains from the United States and Canada and In Silico Identification of Antigenically Important Sites

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Longitudinal Surveillance of Porcine Rotavirus B Strains from the United States and Canada and In Silico Identification of Antigenically Important Sites

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider