November 1, 1987Journal of Biological ChemistryOpen Access

Isolation of GTP-binding proteins from myeloid HL-60 cells. Identification of two pertussis toxin substrates.

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Population

Myeloid HL-60 cell plasma membranes

Design

Preclinical

Authors

RURonald J. UhingHoward Hughes Medical Institute PPPaul PolakisIntarcia Therapeutics (United States)RSRalph SnydermanDuke University

Discussion

Member takes

Implication

Suggests distinct G-protein roles in myeloid cells; leaves open their relevance to human disease.

Structured PICO

Population

Myeloid HL-60 cell plasma membranes

Intervention

Isolation and characterization of GTP-binding proteins (including partial chymotryptic proteolysis and mild acid hydrolysis)

Outcome

Identification and characterization of pertussis toxin substratessurrogate

The study identifies two distinct pertussis toxin substrates in myeloid HL-60 cells, suggesting different functional roles for the 40-kDa and 41-kDa GTP-binding proteins.

Cite This Study

Uhing et al. (1987) studied this question.

synapsesocial.com/papers/6a897941abb0ef83c5c98333 https://doi.org/10.1016/s0021-9258(18)47765-4

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