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MethodsLiver-The main portion of the liver was transferred to a clock glass and cut fine with scissors and then placed in a vacuum drying oven at a temperature of 48'.After about 20 hours, the tissue was ground in a mortar and then returned to the oven until it was completely dry.It was then transferred to a 100 cc.volumetric flask and about 75 cc. of 95 per cent alcohol were added.If the weight of the fresh liver was more than 5 gm., twice as much of 93
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Blatherwick et al. (1933) studied this question.
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