A note on the isolation of small marine algae and flagellates for pure cultures

Increasing interest in the comparative biochemistry of marine algae and protozoa is creating a demand for experimental material in the form of pure, i.e. bacteria-free, cultures. For nutritional and biochemical studies pure cultures are, of course, obligatory; but the number of marine strains available for this sort of work is lamentably small. Indeed, none of the so-called μ flagellates has hitherto been grown in pure culture as far as I know, although there exist pure strains of some of the larger and hardier forms.