What question did this study set out to answer?

To characterize neurite outgrowth and biochemical responses in a novel PC12 subline (PC12D) exposed to cAMP-enhancing agents and nerve growth factor.

January 1, 1987Journal of Neuroscience Research

Neuritic growth from a new subline of PC12 pheochromocytoma cells: Cyclic AMP mimics the action of nerve growth factor

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To characterize neurite outgrowth and biochemical responses in a novel PC12 subline (PC12D) exposed to cAMP-enhancing agents and nerve growth factor.
Treated PC12D pheochromocytoma cells with cAMP-elevating agents (forskolin), nerve growth factor (NGF), epidermal growth factor, phorbol diester, and anti-NGF antiserum.
Measured intracellular cAMP levels, membrane adenylate cyclase activity, neurite ultrastructure, and the activities of tyrosine hydroxylase (TH), acetylcholinesterase (AchE), and ornithine decarboxylase (ODC).
Forskolin and NGF independently stimulated neurite extension within 24 hours in PC12D cells, with forskolin-induced neurites forming growth cones and microtubule arrays that were unaffected by anti-NGF antiserum.
Forskolin, but not NGF, elevated intracellular cAMP and activated membrane adenylate cyclase, while both agents enhanced TH, AchE, and ODC enzyme activities.

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Implication

In vitro study demonstrates cyclic AMP mimics nerve growth factor in driving neurite outgrowth in PC12D cells, suggesting parallel signaling pathways.

Cite This Study

Katoh‐Semba et al. (1987) studied this question.

synapsesocial.com/papers/6a8982cd4de818ad79d440d0 https://doi.org/10.1002/jnr.490170106

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Implication

In vitro study demonstrates cyclic AMP mimics nerve growth factor in driving neurite outgrowth in PC12D cells, suggesting parallel signaling pathways.

Neuritic growth from a new subline of PC12 pheochromocytoma cells: Cyclic AMP mimics the action of nerve growth factor

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Discussion

Implication

Cite This Study

Neuritic growth from a new subline of PC12 pheochromocytoma cells: Cyclic AMP mimics the action of nerve growth factor

Key Points

Discussion

Implication

Cite This Study

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