What question did this study set out to answer?

To clone and characterize human junctional adhesion molecule 3 (JAM3) and identify its cellular binding partners and physiological role in leukocyte adhesion.

December 1, 2001Journal of Biological ChemistryOpen Access

Cloning of Human Junctional Adhesion Molecule 3 (JAM3) and Its Identification as the JAM2 Counter-receptor

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Authors

MAMaria P. ArrateVanderbilt University Medical Center JRJosé M. RodríguezKing's College London TTTuan M. TranThe University of Texas MD Anderson Cancer Center

Discussion

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Implication

In vitro molecular analysis demonstrates that human JAM3 acts as a counter-receptor for JAM2 on T cells, highlighting a novel mechanism for immune cell adhesion and endothelial interaction.

Key Points

To clone and characterize human junctional adhesion molecule 3 (JAM3) and identify its cellular binding partners and physiological role in leukocyte adhesion.
Cloned human JAM3, mapped its gene to chromosome 11q25 across 9 exons, and evaluated its expression across multiple tissues and endothelial cells.
Assessed molecular interactions using JAM3-Fc/JAM2-Fc binding assays, cell-capture experiments in Chinese hamster ovary cells, and static leukocyte adhesion assays.
Performed pull-down experiments and anti-JAM3 immunoblotting on T cells, assessed lymphocyte activation dynamics, and tested soluble JAM3 ectodomain for adhesion blockade.
Cloned human JAM3, which shows 36% identity to JAM2 and 32% to JAM1, with elevated transcript levels detected in the kidney, brain, placenta, and endothelial cells.
Identified JAM3 as the previously uncharacterized 43-kDa counter-receptor that forms a direct heterotypic complex with JAM2 to mediate T-cell adhesion.
Demonstrated that JAM3 expression is up-regulated on peripheral blood lymphocytes following activation and that soluble JAM3 ectodomain blocks JAM2-mediated adhesion.

Cite This Study

Arrate et al. (2001) studied this question.

synapsesocial.com/papers/6a89836d5edafe343d92e4f1 https://doi.org/10.1074/jbc.m105972200

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Implication

In vitro molecular analysis demonstrates that human JAM3 acts as a counter-receptor for JAM2 on T cells, highlighting a novel mechanism for immune cell adhesion and endothelial interaction.

Cloning of Human Junctional Adhesion Molecule 3 (JAM3) and Its Identification as the JAM2 Counter-receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

Cloning of Human Junctional Adhesion Molecule 3 (JAM3) and Its Identification as the JAM2 Counter-receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

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