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May 8, 2014Scientific ReportsOpen Access

Ultrastructural Localization of Adiponectin protein in Vasculature of Normal and Atherosclerotic mice

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Key result

In atherosclerotic mice, adiponectin localized not only to endothelial cells but also to synthetic smooth muscle cells and adherent monocytes, whereas in normal mice it was restricted mainly to endothelial cells.

Authors

TMTakuya MoriKyoto UniversityYKYoshihisa KoyamaPsychiatric Medical CenterNMNorikazu MaedaGeneral / Preventive / Lipids

Discussion

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Implication

May implicate adiponectin in plaque cellular dynamics beyond endothelium; hypothesis-generating for human atherosclerosis studies.

Key Points

  • Investigate the precise ultrastructural localization of adiponectin within the aortic wall of healthy and atherosclerotic mice.
  • Examined aortic tissue samples from wild-type and apolipoprotein E-knockout (ApoE-KO) mice.
  • Mapped the subcellular distribution of adiponectin using immunoelectron microscopy.
  • In wild-type mice, adiponectin localized predominantly to the luminal surface membrane and intracellular endocytic vesicles of endothelial cells.
  • In atherosclerotic ApoE-KO mice, adiponectin was identified in endothelial cells, on the cell surface of synthetic smooth muscle cells, and on adherent monocytes.

PICO

P
Population
Male wild-type, adiponectin knockout, and apolipoprotein E knockout mice were studied to determine the ultrastructural localization of adiponectin in normal and atherosclerotic aortas.
E
Exposure / Comparator
Atherosclerosis (ApoE-KO mice fed Western diet) vs Normal vasculature (WT mice)
O
Primary Outcome
Ultrastructural localization of adiponectin protein in the aorta

Limitations

  • The exact molecular mechanism responsible for adiponectin accumulation in atherosclerotic lesions is unclear.
  • The expression levels of adiponectin-binding molecules in monocytes remain uncertain.
  • It is not clear whether adiponectin binds to monocytes in circulating peripheral blood.

Cite This Study

Mori et al. (2014) studied Atherosclerosis. Atherosclerosis (ApoE-KO mice fed Western diet) vs. Normal vasculature (WT mice) was evaluated on Ultrastructural localization of adiponectin protein in the aorta. In atherosclerotic mice, adiponectin localized not only to endothelial cells but also to synthetic smooth muscle cells and adherent monocytes, whereas in normal mice it was restricted mainly to endothelial cells.

synapsesocial.com/papers/6a8986f3176d2ac34cfba479https://doi.org/10.1038/srep04895
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Evidence for the presence of oxidatively modified low density lipoprotein in atherosclerotic lesions of rabbit and man.1989 · 1,893 citations
  2. 2Adiponectin and Metabolic Syndrome2003 · 1,206 citations
  3. 3Adiponectin Protects Against Angiotensin II–Induced Cardiac Fibrosis Through Activation of PPAR-α2008 · 183 citations
  4. 4Adiponectin Reduces Atherosclerosis in Apolipoprotein E-Deficient Mice2002 · 1,037 citations