Molecular Weight and N-Terminal Amino Acids of the Proteins of the Phosvitin Fraction of Avian Egg Yolk

Molecular weight and N-terminal amino acid analyses of the proteins of egg yolk phosvitin were made using SDS acrylamide disc gel and phenylisothiocyanate techniques, respectively. A total of 18 protein fractions were found with molecular weights ranging from 1.24 × 104 to 1.19 × 105. No proteins were found with molecular weight between 2.18 to 7.18 × 104. Five different N-terminal amino acids were detected throughout 10 sections consisting of 13 bands from preparatory acrylamide gels, namely, alanine, arginine, asparagine, glutamine and threonine.