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Mouse brain tubulin was analyzed on isoelectric focusing gels. High-resolution gels utilizing Bio-Rad ampholytes (pH 4--6) revealed 5--6 bands in the region corresponding to the alpha-subunit of tubulin and 10 or more for the beta-subunit. The same general banding pattern was observed regardless of the method of preparation of the tubulin. Two species prominent in the brains of immature mice, alpha 6 and beta 2, virtually disappeared during maturation, while species beta 6 to beta 10 appeared. No significant changes from the mature pattern were seen during aging (examined at 12, 23, and 30 months of age).
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Hungen et al. (1981) studied this question.
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