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An inexpensive photometric detector system was designed for use with a 96-well microtiter plate. The absorbance of solutions in the wells was measured by using a light-emitting diode as the light source and a Vernier LabPro data-collection interface with a light sensor as the detector. The feasibility of the system was demonstrated with a linear absorbance versus concentration calibration curve constructed from solutions of yellow food coloring. The results of an ELISA experiment for the detection of IgG proteins in rabbit serum confirmed the usefulness of the device for such experiments.
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Gordon et al. (2005) studied this question.
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