Key result
This paper presents a detailed protocol for using Bimolecular Fluorescence Complementation (BiFC) to study protein-protein interactions in Drosophila myofibrils in vivo.
Why the study?
Protein-protein interactions in Drosophila myofibrils are essential for their function and formation, making bimolecular fluorescence complementation an ideal method to study their interactions and spatial distribution.
Enables in vivo BiFC for Drosophila myofibril interactions; extends model-system tools but leaves open translation to mammalian cardiology.
Protein-protein interactions in Drosophila myofibrils are essential for their function and formation. Bimolecular Fluorescence Complementation (BiFC) is an effective method for studying protein interactions and localization. BiFC relies on the reconstitution of a monomeric fluorescent protein from two half-fragments when in proximity. Two proteins tagged with the different half-fragments emit a fluorescent signal when they are in physical contact, thus revealing a protein interaction and its spatial distribution. Because myofibrils are large networks of interconnected proteins, BIFC is an ideal method to study protein-protein interactions in myofibrils. Here we present a protocol for generating transgenic flies compatible with BiFC and a method for analyzing protein-protein interactions based on the fluorescent BiFC signal in myofibrils. Our protocol is applicable to the majority of Drosophila proteins and with few modifications may be used to study any tissue.
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Marescal et al. (2020) studied this question. Bimolecular Fluorescence Complementation (BiFC) was evaluated. This paper presents a detailed protocol for using Bimolecular Fluorescence Complementation (BiFC) to study protein-protein interactions in Drosophila myofibrils in vivo.
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