Experimental Murine Trypanosoma congolense Infections. II. Role of Splenic Adherent CD3+ Thy1.2+ TCR-αβ− γδ− CD4+8− and CD3+ Thy1.2+ TCR-αβ− γδ− CD4−8− Cells in the Production of IL-4, IL-10, and IFN-γ and in Trypanosome-Elicited Immunosuppression

Trypanosome-induced suppression of T and B cell responses to parasite-related and -unrelated Ags is considered a major mechanism of evasion of the host’s immune defenses by the parasite. Reduced T and B cell responses have been attributed to suppressor T cells, suppressor macrophages, or both. We have previously shown that endogenously produced IL-10 and IFN-γ mediate the suppression of T cell responses in Trypanosoma congolense-infected mice. Here, we show for the first time that splenic CD3+ Thy1.2+ αβ− γδ− CD4+8− and CD3+ Thy1.2+ αβ− γδ− CD4−8− cells that copurify with plastic-, nylon wool-, or Sephadex G-10-adherent cell populations, in synergy with adherent Thy1.2− cells, are the major producers of IL-4, IL-10, and IFN-γ in T. congolense-infected mice. We further demonstrate the involvement of these cells in the suppression of T cell proliferative responses to mitogen and in B cell responses to a parasite-unrelated Ag.