Key result
The eNOS gene G894T polymorphism was not significantly associated with pregnancy-induced hypertensive disorders (p > 0.05), although serum NO levels were significantly lower in affected patients.
Why the study?
Is the eNOS gene G894T polymorphism associated with serum nitric oxide levels in patients with pregnancy-induced hypertensive disorders?
Case-Control (n=160)
Is the eNOS gene G894T polymorphism associated with serum nitric oxide levels in patients with pregnancy-induced hypertensive disorders?
Pregnancy-induced hypertension is associated with low serum nitric oxide levels, but not with the eNOS gene G894T polymorphism.
Low NO levels in pregnancy-induced hypertension merit mechanistic study; leaves open eNOS G894T contributions in larger cohorts.
OBJECTIVE: Pregnancy-induced hypertension is one of the most important cause of maternal-fetal morbidity and mortality. Pregnancy-related hypertensive disorders are usually associated with diminished nitric oxide (NO) levels. We aimed to evaluate the role of serum NO levels and eNOS gene G894T polymorphism on hypertensive disorders of pregnancy. METHODS: Eighty patients with gestational hypertension or preeclampsia, and 80 healthy pregnants were enrolled to analyze serum NO levels and G894T polymorphism of the eNOS gene. NO level was analyzed by high-performance liquid chromatography (HPLC) method. The G894T polymorphism of the eNOS gene was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). RESULTS: There was no significant difference between groups in terms of G894T/eNOS genotype and allele frequencies (p > 0.05). Serum NO levels were significantly lower in the patients group. In the control group, subjects with thymine-thymine (TT) genotype had significantly lower NO levels when compared to subjects with guanine-guanine (GG) or guanine-thymine (GT) genotype (p < 0.05). CONCLUSIONS: We failed to demonstrate an association between eNOS gene G894T polymorphism and serum NO levels in patients with pregnancy-induced hypertensive disorders. We established a relation between pregnancy-induced hypertension and low NO levels.
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Sakar et al. (2014) conducted a case-control in Gestational hypertension or preeclampsia (n=160). eNOS gene G894T polymorphism vs. Healthy pregnant women was evaluated on G894T/eNOS genotype and allele frequencies (p=> 0.05). The eNOS gene G894T polymorphism was not significantly associated with pregnancy-induced hypertensive disorders (p > 0.05), although serum NO levels were significantly lower in affected patients.
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