What question did this study set out to answer?

To determine how adrenaline, noradrenaline, isoprenaline, and salbutamol influence the synthesis and release of renin in isolated cat renal cortical cells.

January 1, 1975British Journal of PharmacologyOpen Access

Effects of Adrenaline, Noradrenaline, Isoprenaline and Salbutamol on the Production and Release of Renin by Isolated Renal Cortical Cells of the Cat

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Population

Isolated renal cortical cells of the cat

Comparison

Adrenaline, noradrenaline, isoprenaline, or… vs Incubated controls without added agonist

Design

Preclinical

Follow-up

2 hours

Authors

EJEdward J. JohnsUniversity College Cork HRHugh K. RichardsMonash Health BSBertha SingerMcGill University

Discussion

Member takes

Implication

Distinguishes catecholamine effects on renin in feline cells; hypothesis-generating and requires human validation before any clinical consideration.

Key Points

To determine how adrenaline, noradrenaline, isoprenaline, and salbutamol influence the synthesis and release of renin in isolated cat renal cortical cells.
Isolated feline renal cortical cells were incubated in vitro for 2 hours with or without adrenergic agonists.
Cultures were exposed to noradrenaline (1.18 × 10⁻⁴ M), adrenaline (1.09 × 10⁻⁴ M), isoprenaline (0.72 × 10⁻⁴ M), or salbutamol.
Renin content was measured separately within the cellular fraction and the surrounding incubation medium.
Control cells incubated for 2 h showed a mean 27.2% increase in total renin content, with the increase localized to the incubation medium.
Noradrenaline (1.18 × 10⁻⁴ M) and adrenaline (1.09 × 10⁻⁴ M) stimulated overall renin production by 45% and 34%, respectively, relative to incubated controls.
Isoprenaline (0.72 × 10⁻⁴ M) significantly depleted cellular renin while increasing medium renin without altering total production; salbutamol induced a similar release pattern at roughly one-third the potency of isoprenaline.

Structured PICO

Population

Isolated renal cortical cells of the cat

Intervention

Adrenaline (1.09 x 10^-4 M), noradrenaline (1.18 x 10^-4 M), isoprenaline (0.72 x 10^-4 M), or salbutamol added to incubation medium

Comparator

Incubated controls without added agonist

Outcome

Production and release of reninsurrogate

Adrenaline and noradrenaline stimulate renin production in isolated cat renal cortical cells, whereas isoprenaline and salbutamol only induce its release.

Cite This Study

Johns et al. (1975) studied this question.

synapsesocial.com/papers/6a89a944b4e829b19bd45895 https://doi.org/10.1111/j.1476-5381.1975.tb07331.x

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January 1, 1975British Journal of PharmacologyOpen Access

Effects of Adrenaline, Noradrenaline, Isoprenaline and Salbutamol on the Production and Release of Renin by Isolated Renal Cortical Cells of the Cat

View Full Paper

Ask AI

Bookmark

Population

Isolated renal cortical cells of the cat

Comparison

Adrenaline, noradrenaline, isoprenaline, or… vs Incubated controls without added agonist

Design

Preclinical

Follow-up

2 hours

Authors

EJEdward J. JohnsUniversity College Cork HRHugh K. RichardsMonash Health BSBertha SingerMcGill University

Discussion

Member takes

Implication

Distinguishes catecholamine effects on renin in feline cells; hypothesis-generating and requires human validation before any clinical consideration.

Key Points

To determine how adrenaline, noradrenaline, isoprenaline, and salbutamol influence the synthesis and release of renin in isolated cat renal cortical cells.
Isolated feline renal cortical cells were incubated in vitro for 2 hours with or without adrenergic agonists.
Cultures were exposed to noradrenaline (1.18 × 10⁻⁴ M), adrenaline (1.09 × 10⁻⁴ M), isoprenaline (0.72 × 10⁻⁴ M), or salbutamol.
Renin content was measured separately within the cellular fraction and the surrounding incubation medium.
Control cells incubated for 2 h showed a mean 27.2% increase in total renin content, with the increase localized to the incubation medium.
Noradrenaline (1.18 × 10⁻⁴ M) and adrenaline (1.09 × 10⁻⁴ M) stimulated overall renin production by 45% and 34%, respectively, relative to incubated controls.
Isoprenaline (0.72 × 10⁻⁴ M) significantly depleted cellular renin while increasing medium renin without altering total production; salbutamol induced a similar release pattern at roughly one-third the potency of isoprenaline.

Structured PICO

Population

Isolated renal cortical cells of the cat

Intervention

Adrenaline (1.09 x 10^-4 M), noradrenaline (1.18 x 10^-4 M), isoprenaline (0.72 x 10^-4 M), or salbutamol added to incubation medium

Comparator

Incubated controls without added agonist

Outcome

Production and release of reninsurrogate

Adrenaline and noradrenaline stimulate renin production in isolated cat renal cortical cells, whereas isoprenaline and salbutamol only induce its release.

Cite This Study

Johns et al. (1975) studied this question.

synapsesocial.com/papers/6a89a944b4e829b19bd45895 https://doi.org/10.1111/j.1476-5381.1975.tb07331.x

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