January 1, 1974Proceedings of the National Academy of SciencesOpen Access

Inhibition of Three Nucleotide Polymerases by Rifamycin Derivatives

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Population

E. coli DNA-dependent DNA polymerase, E. coli DNA-dependent RNA polymerase, and viral RNA-instructed DNA…

Design

Preclinical

Authors

FTFrances M. ThompsonTexas Woman's University ATAllan N. TischlerLawrence Berkeley National Laboratory JAJesse W. AdamsNational Institutes of Health

Discussion

Member takes

Implication

It is possible to design rifamycin derivatives that can distinguish between viral RNA-instructed DNA polymerase and other nucleotide polymerases, with higher molecular weight rifazone derivatives showing increased specificity.

Structured PICO

Population

E. coli DNA-dependent DNA polymerase (DDP), E. coli DNA-dependent RNA polymerase (DRP), and viral RNA-instructed DNA polymerase (RDP) from murine leukemia virus (MLV)-transformed UC1-B cells

Intervention

Rifamycin derivatives (14 derivatives tested, including DMB, Octyloxime, Rifamazine, Dirifampin, RC-7, RC-11, RC-13, RC-16, R-42, R-52, R-62, R-82, Rifampicin, RMI)

Outcome

Inhibition constant (KI), defined as the molar concentration of the rifamycin derivative yielding a 50% inhibition of enzyme activitysurrogate

Limitations

Specificity measurements are minimum values because DDP is more highly purified than RDP, and other proteins in the RDP extract may bind the derivatives.

Cite This Study

Thompson et al. (1974) studied this question.

synapsesocial.com/papers/6a89bb5cd89205768aeb4628 https://doi.org/10.1073/pnas.71.1.107

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January 1, 1974Proceedings of the National Academy of SciencesOpen Access

Inhibition of Three Nucleotide Polymerases by Rifamycin Derivatives

View Full Paper

Ask AI

Bookmark

Population

E. coli DNA-dependent DNA polymerase, E. coli DNA-dependent RNA polymerase, and viral RNA-instructed DNA…

Design

Preclinical

Authors

FTFrances M. ThompsonTexas Woman's University ATAllan N. TischlerLawrence Berkeley National Laboratory JAJesse W. AdamsNational Institutes of Health

Discussion

Member takes

Implication

Structured PICO

Population

E. coli DNA-dependent DNA polymerase (DDP), E. coli DNA-dependent RNA polymerase (DRP), and viral RNA-instructed DNA polymerase (RDP) from murine leukemia virus (MLV)-transformed UC1-B cells

Intervention

Rifamycin derivatives (14 derivatives tested, including DMB, Octyloxime, Rifamazine, Dirifampin, RC-7, RC-11, RC-13, RC-16, R-42, R-52, R-62, R-82, Rifampicin, RMI)

Outcome

Inhibition constant (KI), defined as the molar concentration of the rifamycin derivative yielding a 50% inhibition of enzyme activitysurrogate

Limitations

Specificity measurements are minimum values because DDP is more highly purified than RDP, and other proteins in the RDP extract may bind the derivatives.

Cite This Study

Thompson et al. (1974) studied this question.

synapsesocial.com/papers/6a89bb5cd89205768aeb4628 https://doi.org/10.1073/pnas.71.1.107

Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

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