June 14, 2002Science

AID Enzyme-Induced Hypermutation in an Actively Transcribed Gene in Fibroblasts

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Population

NIH 3T3 murine fibroblast cells with an artificial GFP substrate

Design

Preclinical

Authors

KYKiyotsugu YoshikawaDoshisha Women's College of Liberal Arts IOIl‐mi OkazakiThe University of Tokyo TETomonori EtoKyoto University

Discussion

Member takes

Implication

Extends AID-mediated hypermutation to fibroblasts; leaves open B-cell specificity and in vivo relevance.

Structured PICO

Population

NIH 3T3 murine fibroblast cells with an artificial GFP substrate

Intervention

Ectopic expression of Activation-induced cytidine deaminase (AID)

Outcome

Frequency and distribution of mutations in the artificial GFP substratesurrogate

This study demonstrates that AID is sufficient to generate somatic hypermutation in actively transcribed genes in fibroblasts, indicating that necessary cofactors are present outside of B cells.

Cite This Study

Yoshikawa et al. (2002) studied this question.

synapsesocial.com/papers/6a89c407b4e829b19bd46036 https://doi.org/10.1126/science.1071556

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AID Enzyme-Induced Hypermutation in an Actively Transcribed Gene in Fibroblasts

Discussion

Member takes

Implication

Structured PICO

Cite This Study

AID Enzyme-Induced Hypermutation in an Actively Transcribed Gene in Fibroblasts

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider