The N-terminal region of the herpes simplex virus type 1 origin binding protein is essential for its cooperative binding to the viral origin of replication oriS.
N-terminal OBP may represent an HSV antiviral target; leaves open translation beyond animal models.
The origin binding protein (OBP) of herpes simplex virus type 1 is required to activate a viral origin of replication in vivo. We have used intact OBP as well as a truncated form of the protein expressed in Escherichia coli to investigate the protein-protein interactions, as well as the protein-DNA interactions involved in the formation of a nucleoprotein complex at a viral origin of replication (oriS) in vitro. The salient findings demonstrate that the N-terminal part of OBP is required for the cooperative binding of OBP to three sites (boxes I, II, and III) within oriS. A detailed model for the interaction of OBP with the viral origins of replication oriS and oriL is presented.
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Elias et al. (1992) studied this question.
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