A method is described to determine gaschromatographically the individual component of glycosphingolipid after methanolysis and trifluoroacetylation. By using a mixed coated column of SE-30 and XE-60 and under suitable temperature-programmed conditions, neutral hexoses, hexosamines, neuraminic acid, and sphingosine bases from glycolipids were analyzed in one run with considerable accuracy.
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Tamio Yamakawa (1971) studied this question.
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