Why the study?
Fatty liver hemorrhage syndrome causes sudden drops in egg production and substantial losses in the laying hen industry, prompting evaluation of Atractylodes macrocephala Koidz polysaccharide as a protective feedstuff additive.
Does PAMK supplementation ameliorate HELP diet-induced liver injury in laying hens?
Does PAMK supplementation ameliorate HELP diet-induced liver injury in laying hens?
PAMK supplementation at 200 or 400 mg/kg ameliorates high-energy and low-protein diet-induced fatty liver hemorrhagic syndrome in laying hens by regulating antioxidant enzymes and hepatic lipid metabolism.
Structural characterization of PAMK enables targeted FLHS studies; leaves open efficacy and safety in backyard chickens.
Fatty liver hemorrhage syndrome (FLHS) is the most common noninfectious cause of death in backyard chickens worldwide, which can cause a sudden drop in egg production in the affected flocks and cause huge losses to the laying hens breeding industry. In this study, we prepared polysaccharide from Atractylodes macrocephala Koidz (PAMK) by one-step alcohol precipitation. The structural analysis found that PAMK with a molecular weight of 2.816 × 103 Da was composed of glucose and mannose, in a molar ratio of 0.582 to 0.418. Furthermore, we investigated the hepatoprotective effects of PAMK on high-energy and low-protein (HELP) diet–induced FLHS in laying hens. The results showed that the hens' livers of the HELP diet showed yellowish-brown, greasy, and soft, whereas the supplement of PAMK (200 mg/kg or 400 mg/kg) could alleviate such pathological changes. The liver index, the abdominal fat percentage, and liver injury induced by the HELP diet were reduced in PAMK (200 mg/kg or 400 mg/kg). Supplementing 200 mg/kg or 400 mg/kg PAMK showed improvements of the antioxidant capacity in laying hens. Furthermore, we found that the HELP diet increased the expression of hepatic lipogenesis genes and decreased the expression of fatty acid β-oxidation genes, which could be reversed by 200 mg/kg or 400 mg/kg PAMK supplementation. Nevertheless, there is no difference between the addition of 40 mg/kg PAMK and the HELP group. Collectively, these results showed that PAMK supplements could ameliorate HELP diet–induced liver injury through regulating activities of antioxidant enzymes and hepatic lipid metabolism. Therefore, PAMK could be a potential feedstuff additive to alleviate FLHS in laying hens.
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Miao et al. (2020) studied this question.
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