What question did this study set out to answer?

To comprehensively identify and characterize the protein cargo bound to human high-density lipoprotein particles using advanced proteomic technologies.

January 1, 2006PROTEOMICS

Proteomic analysis of high‐density lipoprotein

View Full Paper

Ask AI

Bookmark

Authors

FRFarhad RezaeeUniversity Medical Center Groningen BCBruno CasettaNational Agency for New Technologies, Energy and Sustainable Economic Development JLJ.H.M. LevelsAmsterdam University Medical Centers

Discussion

Member takes

Implication

Proteomic analysis reveals 56 functional proteins associated with human high-density lipoprotein, indicating diverse physiological roles beyond lipid metabolism.

Key Points

To comprehensively identify and characterize the protein cargo bound to human high-density lipoprotein particles using advanced proteomic technologies.
Purified human HDL using a one-step ultracentrifugation protocol to minimize protein loss, verifying particle quality with nephelometry, high-performance gel chromatography, and Western blotting.
Identified HDL-associated proteins using 1-DE and 2-DE MALDI-TOF mass spectrometry alongside isotope-coded affinity tagging and Western blot validation.
Identified 56 distinct proteins associated with human HDL, including all known apolipoproteins and primary lipid transport proteins.
Demonstrated that HDL carries diverse non-lipid proteins, including regulators of hemostasis, thrombosis, complement activation, immune responses, hormones, and growth factors.

Cite This Study

Rezaee et al. (2006) studied this question.

synapsesocial.com/papers/6a89d2ca37ae28c948432380 https://doi.org/10.1002/pmic.200500191

View Full Paper

Ask AI

Bookmark

Implication

Proteomic analysis reveals 56 functional proteins associated with human high-density lipoprotein, indicating diverse physiological roles beyond lipid metabolism.

Proteomic analysis of high‐density lipoprotein

Discussion

Member takes

Implication

Key Points

Cite This Study

Proteomic analysis of high‐density lipoprotein

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider