Provides a comprehensive overview of biochemical analysis methods and functional tests to determine lipid and lipoprotein phenotypes in experimental mouse models.
Standardizes lipid phenotyping in mouse models; leaves open translation to human cardiovascular risk assessment.
Experimental mouse models are widely used for preclinical research on dyslipidemia, atherosclerosis, and cardiometabolic diseases. This unit reports the most commonly used biochemical analysis methods available to determine the lipid and lipoprotein phenotype in the mouse. The discussed methods include: the qualitative and quantitative analysis of lipids, apolipoproteins, and lipoproteins (with a specific emphasis on species-specificities of mice and humans), and the activity assay of major enzymes involved in lipoprotein remodeling (LCAT, PLTP, and LPL). The unit also discusses the most frequently used functional tests to analyze lipid/lipoprotein metabolism in vivo, including triglyceride metabolism, reverse cholesterol transport, intestinal lipid absorption, and secretion. Curr. Protoc. Mouse Biol. 1:265-277 © 2011 by John Wiley & Sons, Inc.
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Tailleux et al. (2011) studied this question.
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