Investigations by VanDemark et al. (12) have shown that high oxygen tensions are detrimental to the livability of bovine spermatozoa in the 3.6 citrateyolk extender or diluter. Presumably, the decreased livability reflects an increased H202 production, as has been demonstrated by Tosie and Walton (11) and Tosic (10). The recent report by Prince and Almquist (5) that agitation of semen in partially filled tubes was harmful to spermatozoan survival also suggests the possibility of oxygen damage due to excessive aeration.
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Vandemark et al. (1950) studied this question.
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