The genomic DNA molecule of an Israeli isolate of tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, was amplified from total DNA extracts of TYLCV-infected plants (Lycopersicon esculentum 'M82') by the use of the polymerase chain reaction (PCR). Synthetic oligonucleotides complementary to different regions of the viral genome were used in different combinations to amplify the full-length as well as subgenomic fragments of the TYLCV DNA molecule (...)
No takes yet. Share an insight, caveat, or question.
N. Navot (1992) studied this question.