Comparative Analysis of the Productivity and Immunogenicity of an Attenuated Classical Swine Fever Vaccine (LOM) and an Attenuated Live Marker Classical Swine Fever Vaccine (Flc-LOM-BErns) from Laboratory to Pig Farm

Figures

• 

  Figure 1. Comparison of the weight and food intake between pigs vaccinated with low virulent of Miyagi (LOM) + swine erysipelothrix rhusiopathiae (SE) or Flc-LOM-BErns + SE at the Animal and Plant Quarantine Agency (APQA) laboratory. Body weight and age (A), food intake per day (B), and intake rate (C). The LOM + SE and Flc-LOM-BErns + SE vaccines are shown in black and red, respectively. ** p < 0.01.

• 

  Figure 2. Comparison of slaughter age (days) between pigs vaccinated with LOM + SE or Flc-LOM-BErns + SE at the APQA laboratory and pig farm (Pungil (PI)). Data represent the mean ± standard error.

• 

  Figure 3. Antibodies induced by the LOM + SE and Flc-LOM-BErns + SE vaccines at the APQA laboratory. Classical swine fever (CSF) E2 antibodies (A), serum neutralizing antibodies (B), CSF Erns antibodies (C), and bovine viral diarrhea (BVD) Erns antibodies (D). The LOM + SE and Flc-LOM-BErns + SE vaccines are shown in black and red dots, respectively. CSFV, classical swine fever virus. PC (positive control) and S/P ratio are positive control and sample O.D/positive control O.D, respectively.

• 

  Figure 4. Changes in the body temperature and leukocyte counts post-vaccination. Body temperature (A) and leukocyte counts (B). The LOM + SE and Flc-LOM-BErns + SE vaccines are shown in light gray and white bars, respectively (A). The LOM + SE, Flc-LOM-BErns + SE, and mock vaccines (no vaccine) are shown in black, red, and white dots, respectively (B). Data represent the mean ± standard error.

• 

  Figure 5. Expression of cytokines (IFN-γ, IL-10, TNF-α, and IL-6) at seven days post-vaccination. Relative mRNA expression was calculated using the 2−ΔΔCt method. The LOM + SE, Flc-LOM-BErns + SE, and mock groups are denoted by gray, light gray, and black bars, respectively. * p < 0.05.

• 

  Figure 6. Food intake by pigs vaccinated with the LOM + SE and Flc-LOM-BErns + SE vaccines on a pig farm. Food intake per day (A) and intake rate (B). The LOM + SE and Flc-LOM-BErns + SE vaccines are denoted by black and red, respectively. * p < 0.05 and ** p < 0.01.

• 

  Figure 7. Antibodies induced by the two CSF vaccines used on the pig farm. CSF E2 antibodies (A), serum neutralizing antibodies (B), CSF Erns antibodies (C), and BVD Erns antibodies (D). The LOM + SE and Flc-LOM-BErns + SE vaccines are marked in black and red, respectively. Data represent the mean ± standard error and ** indicates significant differences (p < 0.001).