April 26, 2002Biochemistry

Role of Basic Residues of the Autolysis Loop in the Catalytic Function of Factor Xa

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Population

Mammalian cells expressing mutant fX cDNA

Comparison

Substitution of basic residues (Arg, Lys, Arg… vs Wild-type factor Xa

Design

Preclinical

Authors

CMChandrashekhara ManithodySaint Louis University LYLikui YangHebei GEO University ARAlireza R. RezaieUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Arg(150) mutation impairs antithrombin reactivity with factor Xa; leaves open its role in anticoagulation mechanisms pending in vivo validation.

Structured PICO

Population

Mammalian cells expressing mutant fX cDNA

Intervention

Substitution of basic residues (Arg(143), Lys(147), Arg(150), and Arg(154)) individually with Ala in the fX cDNA

Comparator

Wild-type factor Xa

Outcome

Ability to assemble into the prothrombinase complex to activate prothrombin and interact with target plasma fXa inhibitors (TFPI and antithrombin)surrogate

Arg(150) of the autolysis loop of factor Xa may specifically interact with the activated conformation of antithrombin.

Cite This Study

Manithody et al. (2002) studied this question.

synapsesocial.com/papers/6a8a5d5cd513dfc69d721a40 https://doi.org/10.1021/bi0255367

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Implication

Arg(150) mutation impairs antithrombin reactivity with factor Xa; leaves open its role in anticoagulation mechanisms pending in vivo validation.

Role of Basic Residues of the Autolysis Loop in the Catalytic Function of Factor Xa

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Role of Basic Residues of the Autolysis Loop in the Catalytic Function of Factor Xa

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider