Various effects of 11,12 EET rescue wound healing in a combined model of diabetes and ischemia

Figure 1. (a) Photograph of the wound on the mouse ear. Black arrows indicating the two ligated vessels. (b) Day of wound closure. Comparison between non-treated and 11,12 EET treated ischemic wounds in diabetic mice. (c) Percentage of closed wound area from day 0–16. Comparison between ischemic wounds of diabetic animals with or without 11,12 EET treatment. (d) Representative pictures of ischemic wounds throughout the wound healing process in diabetic mice and after treatment with 11,12 EET (data is shown as mean ± SD; n = 10). **p < 0.01, ***p < 0.001.

Figure 2. (a) Percentage of TNF-α positive area on day 3, 6, 9, and 18 of ischemic wounds of diabetic mice with 11,12 EET treatment and solvent control; on the right, representative pictures of the staining. (b) Percentage of TGF-β positive cells on day 3, 6, 9, and 18 of ischemic wounds of diabetic mice with 11,12 EET treatment and solvent control, representative pictures of the staining (data is shown as mean ± SD; n = 8 for day 3,6, an 9 n = 10 for day 18). *p < 0.05, **p < 0.01.

Figure 3. (a) Percentage of SDF-1α positive cells on day 3, 6, 9, and 18 of ischemic wounds of diabetic mice with 11,12 EET treatment and solvent control; on the right, representative pictures of the staining (n = 12). (b) Percentage of Ki-67 positive area on day 3, 6, 9, and 18 of ischemic wounds of diabetic mice with 11,12 EET treatment and solvent control; on the right, representative pictures of the staining (data is shown as mean ± SD; n = 8 for day 3,6, an 9 n = 10 for day 18). **p < 0.01.

Figure 4. (a) Percentage of VEGF positive area on day 3, 6, 9, and 18 of ischemic wounds of diabetic mice with 11,12 EET treatment and solvent control; on the right, representative pictures of the staining. (b) Percentage of CD31 positive cells on day 3, 6, 9, and 18 of ischemic wounds of diabetic mice with 11,12 EET treatment and solvent control; on the right, representative pictures of the staining (data is shown as mean ± SD; n = 8 for day 3,6, an 9 n = 10 for day 18). *p < 0.05, **p < 0.01, ***p < 0.001.