What question did this study set out to answer?

To determine whether mutations in the conserved LSGGQ motif of the ABC transporter Ste6p impair a-factor export and directly affect mating efficiency in Saccharomyces cerevisiae.

March 1, 1996Journal of BacteriologyOpen Access

Mutations within the first LSGGQ motif of Ste6p cause defects in a-factor transport and mating in Saccharomyces cerevisiae

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Authors

BBB L BrowneUniversity of Oregon VMV. McClendonUniversity of Alabama at Birmingham DBDavid M. BedwellUniversity of Alabama at Birmingham

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Implication

Mutational analysis demonstrates that Ste6p LSGGQ motif alterations impair a-factor export and mating in Saccharomyces cerevisiae, highlighting this conserved domain's direct role in ABC transport.

Key Points

To determine whether mutations in the conserved LSGGQ motif of the ABC transporter Ste6p impair a-factor export and directly affect mating efficiency in Saccharomyces cerevisiae.
Engineered missense mutations within and adjacent to the first LSGGQ motif located in the nucleotide-binding domain of Ste6p in Saccharomyces cerevisiae.
Quantified extracellular a-factor pheromone levels, mating efficiency, Ste6p steady-state protein expression, and subcellular localization.
Mutations directly within the LSGGQ motif produced the most severe reductions in extracellular a-factor transport.
Extracellular a-factor levels strongly correlated with mating efficiency across all mutant yeast strains.
Mutations did not alter Ste6p steady-state expression levels or subcellular localization, indicating a specific defect in transport activity.

Cite This Study

Browne et al. (1996) studied this question.

synapsesocial.com/papers/6a8a67aaa90c2f4c44fbbbbe https://doi.org/10.1128/jb.178.6.1712-1719.1996

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Implication

Mutational analysis demonstrates that Ste6p LSGGQ motif alterations impair a-factor export and mating in Saccharomyces cerevisiae, highlighting this conserved domain's direct role in ABC transport.

Mutations within the first LSGGQ motif of Ste6p cause defects in a-factor transport and mating in Saccharomyces cerevisiae

Discussion

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Implication

Key Points

Cite This Study

Mutations within the first LSGGQ motif of Ste6p cause defects in a-factor transport and mating in Saccharomyces cerevisiae

Discussion

Member takes

Implication

Key Points

Cite This Study

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