What question did this study set out to answer?

To examine sequence variation within the polyQ-encoding region of the AIB1 gene at the single-molecule level and determine whether these sequence variations relate to AIB1 gene amplification in breast cancer cell lines.

May 2, 2006BMC CancerOpen Access

AIB1 gene amplification and the instability of polyQ encoding sequence in breast cancer cell lines

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To examine sequence variation within the polyQ-encoding region of the AIB1 gene at the single-molecule level and determine whether these sequence variations relate to AIB1 gene amplification in breast cancer cell lines.
Analyzed the polymorphic polyQ-encoding region of AIB1 at the single-molecule level via PCR cloning and sequencing across multiple breast cancer cell lines.
Measured AIB1 gene amplification in estrogen receptor (ER)-positive, ER-negative, and drug-resistant MCF-7-derived cell lines using real-time quantitative PCR.
AIB1 gene amplification (5- to 23-fold) occurred in 2 of 9 (22%) ER-positive lines (MCF-7 and BT-474) and 0% of ER-negative lines, persisting across endocrine-resistant MCF-7 derivatives.
Approximately 72% of breast cancer cell lines exhibited somatic instability characterized by low-frequency (<20%) secondary polyQ sequence variants derived from the parental allele.
Specific polyQ sequence patterns and the occurrence of somatic sequence instability did not correlate with the presence or magnitude of AIB1 gene amplification.

Authors

LWLee-Jun WongBaylor College of Medicine PDPu DaiBeijing Institute of Technology JLJyh‐Feng LuFu Jen Catholic University

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Implication

In vitro study reveals widespread polyQ sequence instability independent of AIB1 gene amplification in breast cancer cell lines, indicating somatic mutation mechanisms during tumor progression.

Cite This Study

Wong et al. (2006) studied this question.

synapsesocial.com/papers/6a8a7459176d2ac34cfbde0e https://doi.org/10.1186/1471-2407-6-111

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Implication

In vitro study reveals widespread polyQ sequence instability independent of AIB1 gene amplification in breast cancer cell lines, indicating somatic mutation mechanisms during tumor progression.

AIB1 gene amplification and the instability of polyQ encoding sequence in breast cancer cell lines

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Implication

Cite This Study

AIB1 gene amplification and the instability of polyQ encoding sequence in breast cancer cell lines

Key Points

Discussion

Implication

Cite This Study

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