The complete 5,812 base pairs nucleotide sequence has been determined for Bacillus subtilis (natto) plasmid pUH1, which encodes a ymglutamyltranspeptidase (EC 2.3.2.2) for synthesis of y-polyglutamate. Nucleotide sequence analysis reveals two long open reading frames coding for the gene for replication (rep) protein and y-glutamyltranspeptidase gene. Several putative regulatory sequences conserved were found upstream from these frames including the ShinemDalgarno sequence, Pribnow box and the “-35 region” at preferred distances for efficient transcription. The amino acid sequences predicted from the rep and ymglutamyltranspeptidase coding frames showed a significant homology, in each small segment, with those of E229 coded from the plasmid PC194 and of C403 from the plasmid pE194 of Staphylococcus aureus, respectively. However, no convincing homology of the pUH1 predicted ymglutamyltranspeptidase protein could be found with E. coli and mammalian ymglutamyltranspeptidases.
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HARA et al. (1992) studied this question.