Optimization of Separation and Determination of Hydroxystilbazole Benzyl Derivatives by Itp Technique

Benzyl derivatives of hydroxystilbazole show biological activity appearing as antimicrobial and anticancer effect. Moreover, these derivatives show properties of liquid crystals and can form complexes. In this paper are presented optimum conditions of separation and determination of two isomers, derivatives of hydroxystilbazole by a technique of isotachophoresis. (E)-N-(benzyl)-4′-hydroxystilbazole bromide and (E)-N-(benzyl)-2′-hydroxystilbazole bromides were subjected to an optimization process. Lengths of analysis steps, electric current intensity and pH of electrolyte solutions and samples were changed during the optimization of conditions. The shortest time of analysis was obtained during determination of individual isomers. However, in the case of mixtures, the time of analysis was lengthened two times and was at about 420 seconds. It was proven by a process of trial and error that optimum pH for analyzed isomers are 3.7.