Experimental study reveals active reperfusion with squats enhances high-intensity running in soccer players, suggesting specialized preconditioning boosts athletic output.
This study aimed to determine whether active ischemic preconditioning, incorporating exercise during the reperfusion phases, would enhance Yo–Yo Intermittent Recovery Test Level 2 performance in soccer players. Seventeen male amateur soccer players (24.0±1.8 y) completed three different preconditioning sessions before the Yo–Yo Intermittent Recovery Test Level 2. The ischemic preconditioning protocol consisted of three cycles of 4-minute occlusion (220 mm Hg), followed by 4-minute reperfusion (0 mm Hg) in both thighs (at rest). Active protocols were similar to ischemic preconditioning; however, during reperfusion, participants performed intermittent running (ischemic preconditioning+Yo–Yo Intermittent Recovery Test Level 2) or three-repetition maximum squat repetitions (ischemic preconditioning+SQUAT). We assessed countermovement jump and Yo–Yo Intermittent Recovery Test Level 2 performance, heart rate, muscle oxygen saturation, blood lactate, rating of perceived exertion, and session-rating of perceived exertion. Ischemic preconditioning+SQUAT resulted in a greater Yo–Yo Intermittent Recovery Test Level 2 distance compared to ischemic preconditioning (p=0.02 and effect size=0.3) and ischemic preconditioning+YOYO (p=0.01, effect size=0.2). Countermovement jump performance was affected by condition (p<0.01), with ischemic preconditioning+YOYO presenting higher values compared with ischemic preconditioning (effect size=1.1), although no time effect was observed. No significant differences were observed for muscle oxygen saturation (p=0.09), blood lactate (p=0.32), and rating of perceived exertion/session-rating of perceived exertion (p=0.96); however, the mean heart rate was higher with active reperfusion conditions than ischemic preconditioning. Active reperfusion combined with heavy squat exercise slightly improved high-intensity intermittent running performance in soccer players, while also eliciting higher heart rate responses, which should be considered during training monitoring.
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Santos et al. (2026) studied this question.
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