May 1, 2008Journal of VirologyOpen Access

Small Interfering RNAs That Deplete the Cellular Translation Factor eIF4H Impede mRNA Degradation by the Virion Host Shutoff Protein of Herpes Simplex Virus

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Population

HeLa cells

Comparison

Depletion of eIF4H by transfection with small… vs Cells transfected with an siRNA for the…

Design

Preclinical

Follow-up

5 hours after infection

Authors

SNS. NandiniUniversidad Nacional Autónoma de México DADeepali AgarwalUniversity of Missouri–Kansas City LSLora A. ShiflettNew York University

Discussion

Member takes

Implication

No change to HSV management; leaves open eIF4H as antiviral target for future study.

Structured PICO

Population

HeLa cells

Intervention

Depletion of eIF4H by transfection with small interfering RNAs (siRNAs) 48 h prior to herpes simplex virus (HSV) infection or mock infection in the presence of actinomycin D

Comparator

Cells transfected with an siRNA for the housekeeping enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

Outcome

Cellular mRNA levels (specifically beta-actin mRNA levels) assayed 5 h after infectionsurrogate

Depletion of eIF4H impedes Vhs-mediated mRNA degradation in HSV-infected cells, suggesting eIF4H binding is required for Vhs activity.

Cite This Study

Nandini et al. (2008) studied this question.

synapsesocial.com/papers/6a8a813f1e528df4f2ec7529 https://doi.org/10.1128/jvi.00137-08

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Small Interfering RNAs That Deplete the Cellular Translation Factor eIF4H Impede mRNA Degradation by the Virion Host Shutoff Protein of Herpes Simplex Virus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Small Interfering RNAs That Deplete the Cellular Translation Factor eIF4H Impede mRNA Degradation by the Virion Host Shutoff Protein of Herpes Simplex Virus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider