Advances in Lipid Digestion and Bioaccessibility Using the INFOGEST 2.0 Protocol

The INFOGEST 2.0 protocol is currently recognized as a reference method for in vitro digestion studies. Its successive improvements have strengthened interlaboratory reproducibility and enabled a more physiologically relevant simulation of gastrointestinal processes. An increasing number of studies have applied the INFOGEST 2.0 model to a wide range of food systems, from simplified ingredient/molecule dispersions to structurally complex foods, demonstrating its relevance for investigating nutrient bioaccessibility across diverse matrix organizations. Lipid digestion has received particular attention because it is strongly governed by supramolecular structure and food matrix complexity. Neutral lipids and polar lipids display distinct interfacial behaviors, digestion kinetics, and micellization patterns, which directly influence the extent of lipolysis and the bioaccessibility of fatty acids. This mini‐review summarizes recent studies applying the INFOGEST 2.0 protocol to lipid digestion in both simple and complex food systems. It highlights the analytical approaches used to quantify lipolysis and lipid bioaccessibility, discusses current methodological challenges of the model, and outlines perspectives for improving the evaluation of lipid digestion in heterogeneous food matrices. Practical applications : This mini‐review provides an overview of the application and recent developments of the INFOGEST 2.0 protocol, with a particular focus on lipid digestion. It serves as a useful resource for researchers seeking to better understand current practices, methodological advances, and the use of the protocol for evaluating lipid digestibility and bioaccessibility in diverse food systems.