Chlorophyf l‐free preparations of plasma membranes from leaves of barley (Hordeum vulgare L. cv. Kristina) and spinach (Spinada oleracea L. cv. Viking II) were obtained by partition in an aqueous dextran‐polyethylene glycol two‐phase system. CJlu‐can synthetase II (EC 2.4,1.34), a marker for the plasma membrane, was highly enriched in both preparations. Silicotungstic acid, a specific stain for the plasma membrane, indicated a purity close to 100% for the barley preparation. Both plasma membrane preparations contained a light‐reducible b‐cytochrome, as shown by low temperature spectroscopy. The plasma membranes had a tow protein content compared to the bulk of intracellular membranes. The polypeptide composition of the barley and spinach plasma membranes showed striking similarities, with.the most prominent polypeptides in the 49‐58 kdalton region, and some further prominent bands in the 30 kcialton region. Some high molecular weight polypeptides in the 73‐110 kdalton region were also typical for the plasma membranes compared to the microsomal fractions.
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Kjellbom et al. (1984) studied this question.
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