What question did this study set out to answer?

To determine how the flagellar master regulator FlhDC controls extracellular protein production and pathogenicity in Erwinia carotovora subsp. carotovora.

April 26, 2008Journal of Bacteriology

Regulatory Network Controlling Extracellular Proteins inErwinia carotovorasubsp.carotovora: FlhDC, the Master Regulator of Flagellar Genes, ActivatesrsmBRegulatory RNA Production by AffectinggacAandhexA(lrhA) Expression

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Authors

YCYaya CuiUniversity of Missouri ACAsita ChatterjeeUniversity of Missouri ACAsita ChatterjeeUniversity of Missouri

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Implication

Experimental study demonstrates FlhDC regulates exoprotein production in Erwinia carotovora, indicating master flagellar regulators coordinate plant virulence.

Key Points

To determine how the flagellar master regulator FlhDC controls extracellular protein production and pathogenicity in Erwinia carotovora subsp. carotovora.
Generated and evaluated transposon-insertion mutants for the flhDC operon, alongside an rsmA flhDC double mutant and plasmid complementation lines carrying flhDC(+) or rsmB(+) DNA.
Assayed extracellular enzyme activity (pectate lyase, polygalacturonase, cellulase, protease), Harpin production, and plant virulence, and performed transcript analyses of gacA, hexA, rsmC, fliA, and rsmB RNA.
FlhDC deficiency severely decreased the secretion of pectate lyase, polygalacturonase, cellulase, protease, and Harpin(Ecc), resulting in strongly attenuated plant virulence that was fully rescued by flhDC(+) plasmid complementation.
FlhDC positively regulated gacA, rsmC, and fliA transcription while repressing hexA; reduced GacA and elevated HexA levels in FlhDC(-) mutants collectively suppressed rsmB noncoding RNA synthesis.
The exoprotein deficiency in FlhDC(-) mutants was mediated by unsequestered RsmA RNA-binding protein and was reversed by deleting rsmA or overexpressing rsmB, whereas fliA regulation had no impact on exoprotein levels.

Cite This Study

Cui et al. (2008) studied this question.

synapsesocial.com/papers/6a8a9b565c716ca1a5d47d4e https://doi.org/10.1128/jb.01828-07

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Implication

Experimental study demonstrates FlhDC regulates exoprotein production in Erwinia carotovora, indicating master flagellar regulators coordinate plant virulence.

Regulatory Network Controlling Extracellular Proteins inErwinia carotovorasubsp.carotovora: FlhDC, the Master Regulator of Flagellar Genes, ActivatesrsmBRegulatory RNA Production by AffectinggacAandhexA(lrhA) Expression

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Key Points

Cite This Study

Regulatory Network Controlling Extracellular Proteins inErwinia carotovorasubsp.carotovora: FlhDC, the Master Regulator of Flagellar Genes, ActivatesrsmBRegulatory RNA Production by AffectinggacAandhexA(lrhA) Expression

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