Pseudomonas fluorescens Pf-5 protected cucumber from preemergence damping-off caused by Pythium ultimum and produced a number of antifungal metabolites in culture, including a fluorescent siderophore (pyoverdine), pyoluteorin, pyrrolnitrin, cyanide, and an uncharacterized compound termed antibiotic 3. Of these, the roles of pyoluteorin and pyoverdine in the biocontrol activity of Pf-5 against Pythium damping-off of cucumber were investigated. Tn5 insertion mutants of Pf-5 were screened for loss of pyoluteorin production (Plt - ) or pyoverdine production (Pvd - ) (...)
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J. Kraus (1992) studied this question.