What question did this study set out to answer?

To determine whether the MprF homolog lpiA in the gram-negative bacterium Rhizobium tropici mediates the synthesis of lysyl-phosphatidylglycerol and contributes to peptide resistance under acidic conditions.

October 8, 2007Molecular Plant-Microbe InteractionsOpen Access

The Lipid Lysyl-Phosphatidylglycerol Is Present in Membranes of Rhizobium tropici CIAT899 and Confers Increased Resistance to Polymyxin B Under Acidic Growth Conditions

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Authors

CSChristian SohlenkampUniversidad Nacional Autónoma de México KGKanaan A. Galindo-Lagunas ZGZiqiang GuanUniversity of New Hampshire

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Implication

In vitro bacterial study demonstrates low-pH induction of lysyl-phosphatidylglycerol in Rhizobium tropici, indicating an adaptive mechanism against cationic peptides.

Key Points

To determine whether the MprF homolog lpiA in the gram-negative bacterium Rhizobium tropici mediates the synthesis of lysyl-phosphatidylglycerol and contributes to peptide resistance under acidic conditions.
Expressed the R. tropici lpiA gene heterologously in Escherichia coli and Sinorhizobium meliloti to evaluate lipid production.
Analyzed lipid profiles of wild-type, lpiA-deletion, and complemented R. tropici CIAT899 strains cultured across acidic (pH 4.5) and neutral conditions in minimal and complex media.
Assessed susceptibility of wild-type and lpiA-deficient R. tropici strains to the cationic antimicrobial peptide polymyxin B under acidic growth conditions.
Heterologous expression of R. tropici lpiA in E. coli and S. meliloti successfully drove the biosynthesis of lysyl-phosphatidylglycerol (LPG).
Wild-type R. tropici synthesized LPG to approximately 1% of total membrane lipids specifically when grown in minimal medium at pH 4.5, whereas lpiA-deficient mutants lacked LPG entirely unless complemented with functional lpiA.
Under acidic growth conditions, lpiA-deficient mutants exhibited greater than fourfold increased susceptibility to polymyxin B relative to the wild-type strain.

Cite This Study

Sohlenkamp et al. (2007) studied this question.

synapsesocial.com/papers/6a8a9cd1ec2e105e8ac15863 https://doi.org/10.1094/mpmi-20-11-1421

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Implication

In vitro bacterial study demonstrates low-pH induction of lysyl-phosphatidylglycerol in Rhizobium tropici, indicating an adaptive mechanism against cationic peptides.

The Lipid Lysyl-Phosphatidylglycerol Is Present in Membranes of Rhizobium tropici CIAT899 and Confers Increased Resistance to Polymyxin B Under Acidic Growth Conditions

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Implication

Key Points

Cite This Study

The Lipid Lysyl-Phosphatidylglycerol Is Present in Membranes of Rhizobium tropici CIAT899 and Confers Increased Resistance to Polymyxin B Under Acidic Growth Conditions

Discussion

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Implication

Key Points

Cite This Study

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