What question did this study set out to answer?

Determine whether de-epithelialized human amnion membrane can support guided neurite outgrowth in vitro and bridge axonal regeneration across lesions in the adult mammalian brain.

May 29, 1987Science

Human Amnion Membrane Serves as a Substratum for Growing Axons in Vitro and in Vivo

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Authors

GDGeorge E. DavisUniversity of South Florida SBScott N. BlakerUniversity of California, San Diego EEEva EngvallLinköping University

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Implication

Preclinical study demonstrates that human amnion membrane bridges promote axonal regeneration across brain lesions in rats, suggesting a viable scaffold for central nervous system repair.

Key Points

Determine whether de-epithelialized human amnion membrane can support guided neurite outgrowth in vitro and bridge axonal regeneration across lesions in the adult mammalian brain.
Prepared human amnion membrane by peeling epithelial cells while leaving the basement membrane and stroma morphologically intact.
Cultured embryonic chick ciliary ganglion motor neurons on membrane cross-sections immunoreactive for laminin.
Implanted rolled amnion membranes longitudinally into surgical lesion cavities between the septum and hippocampus in adult rats.
Embryonic motor neurons attached to both surfaces but grew extensive neurites selectively along laminin-immunoreactive basement membrane pathways.
Implanted rolled membranes facilitated host cholinergic neurons extending regenerating axons directly through the lesion site into the hippocampus.

Cite This Study

Davis et al. (1987) studied this question.

synapsesocial.com/papers/6a8aa0c3755d28965dfd7638 https://doi.org/10.1126/science.3576223

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Implication

Preclinical study demonstrates that human amnion membrane bridges promote axonal regeneration across brain lesions in rats, suggesting a viable scaffold for central nervous system repair.

Human Amnion Membrane Serves as a Substratum for Growing Axons in Vitro and in Vivo

Discussion

Member takes

Implication

Key Points

Cite This Study

Human Amnion Membrane Serves as a Substratum for Growing Axons in Vitro and in Vivo

Discussion

Member takes

Implication

Key Points

Cite This Study

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