Non-muscle myosin 2 filaments are processive in cells

Figures

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  Fig. 1.. Processive-like anterograde movements by RLC (A-E) CAD cell expressing RLC-iRFP. Purple translucent regions in (A) indicate ROI for (C) and (D). B) Cartoon depicting location of cell body and leading edge and direction of retrograde (dotted blue arrow) and anterograde (dotted orange arrow) movements. C) Time-lapse of anterograde (orange highlight) and retrograde (blue highlight) motion of RLC puncta. Partitioning filaments indicated by green arrows. D) Duplicate kymographs with (right) or without (left) retrograde and anterograde movements indicated. E) Histograms of velocity measurements from automated tracking of RLC-iRFP retrograde and anterograde movements in protrusions (see Table 1). F and G) CAD cell expressing RLC-iRFP (magenta) and FTractin-mScarlet (grey). Blue translucent ROI in (F) indicates region used for time-lapse in (G). G) Selected time points demonstrating retrograde (blue highlight) and anterograde (orange highlight) motion along an actin bundle.

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  Fig. 2.. Processive-like Anterograde Movements by Filamentous NM2A. A-C) A representative CAD cell expressing EGFP-NM2A. Purple shaded boxes indicate ROIs for (B) and (C). B) Time-lapse of anterograde moving NM2 filament (orange highlight) that partitions twice (green arrows). C) Kymograph of EGFP-NM2A (left) with retrograde (blue dotted lines) and anterograde (orange dotted lines) movements indicated (right). Blue arrow and orange arrow below left kymograph indicate cell body and leading edge, respectively. D) CAD cell expressing EGFP-NM2A (magenta) and FTractin-mScarlet (grey). Purple shaded boxes indicate ROIs for (E), (F) and (G). E) NM2A filament clearly localizing at a protrusive actin tip. F) NM2A filaments accumulating along actin bundles. G) Time-lapse anterograde movements of NM2A (orange highlight). H) Cartoon of N-terminal EGFP tag on NM2A monomer, filament, and filament imaged with high resolution microscopy.

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  Fig. 3.. NM2 anterograde movements independent of actin dynamics A) Cartoon of C-terminal HaloTag on an NM2 monomer, filament, and imaged with high resolution microscopy. B and D) CAD cell expressing NM2A-Halo imaged before (B) or after (D) treatment with jasplakinolide/latrunculin (JL) cocktail. Purple shaded boxes indicate ROIs for (C) and (E). Magenta arrows indicate NM2 at apparent tips of leading edge actin bundles. C and E) Kymographs shown with (right) and with (left) retrograde (blue dotted lines) and anterograde (orange dotted lines) movements indicated. F) Rose plot of individual retrograde (blue) and anterograde (orange) tracks detected in a single cell before and after JL treatment. G) Ratio of NM2A-Halo intensity within 1 um of the leading edge 45 seconds post-JL treatment relative to last time point before treatment.

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  Fig. 4.. Processive NM2 is dependent on lamellipodia architecture NM2A-Halo was imaged in PFN1-KO CAD cells, CAD cells expressing GFP, or CAD cells overexpressing GFP-PFN1. A-F) Representative images (A, C, E) and duplicate kymographs (B, D, F) with (right) and without (left) annotation of anterograde (orange dotted lines) and retrograde (blue dotted lines) NM2A movements in PFN1 KO (A,B), ctrl + GFP (C,D) and ctrl+ GFP-PFN1 (E,F) cells. G,H) Histograms of velocity measurements from automated tracking of NM2A-Halo puncta in control and PFN1 overexpressing cells (see Table 1). I) Plot of the number of NM2A-Halo anterograde movements in the lamellipodia protrusions of PFN1 KO, control, and PFN1 overexpressing cells. Only anterograde events that initiated within 8 µm of the leading edge were counted. Each dot indicates one cell. Error bars depict mean +/− SD. J) Number of NM2A-Halo puncta within 1 µm of the leading edge of PFN1 KO, control, and PFN1 overexpressing cells. Each dot indicates one cell. Error bars depict mean +/− SD.

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  Fig. 5.. Differential expression, localization and velocity of NM2 isoforms A) Relative RNA expression of NM2A MHC genes. B) Whole cell lysates of untransfected CAD cells or cells over-expressing EGFP-NM2A or -NM2B were subject to western blotting with the indicated antibody. The NM2A:NM2B ratio was calculated using anti-GFP blot as intermediate (see Methods). C) CAD cells fixed and immunostained with indicated NM2 isoform-specific antibody and phalloidin (F-actin). Box in top row indicates ROI for insets in bottom row, which includes merge (rightmost panel). D-I) CAD cells expressing NM2A-Halo (D-F) or NM2B-Halo (G-I). Blue boxes indicate ROI for kymographs in (E) and (H). E and H) Kymographs shown with (right) and without (left) retrograde (blue dotted lines) and anterograde (orange dotted lines) movements indicated. F and I) Histograms of velocity measurements from automated tracking of NM2A-Halo and NM2B-Halo retrograde and anterograde movements in protrusions (see Table 1).

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  Fig. 6.. Processive EGFP-NM2A in fibroblasts A-D) Primary EGFP-NM2A MEF cells imaged with total internal reflection fluorescence structured-illumination microscopy (subnuclear stress fibers; A and B) or Airyscan (ventral stress fibers; C and D). Purple boxes in (A) and (C) indicate ROI for time-lapses in (B) and (D), where processive movements are indicated with orange highlight.