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We have investigated the biological activity in the filtered culture supernatants from 9 VT-producing Escherichia coli strains. The filtrates from 4 strains (3 of human and one of bovine origin), were cytotoxic on Vero and HeLa cells, and caused death in intraperitoneally injected adult mice. The 5 strains of porcine origin showed cytotoxic activity on Vero and Y-1 cells but not on HeLa cells. Filtrates of these latter strains were not lethal for adult mice. VT-cytotoxins produced by all strains were inactive in the infant mouse test and the filtrates from 7 of 8 VT-producing strains assayed in rabbit ileal loops caused fluid accumulation in at least one of the 3 rabbits employed.
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Blanco et al. (1983) studied this question.
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