What question did this study set out to answer?

To isolate chymotryptic peptides and determine the complete amino acid sequence of the amino-terminal segment of the alpha2-CB3 fragment in chick skin collagen.

February 1, 1977European Journal of BiochemistryOpen Access

Covalent Structure of Collagen: Isolation of Chymotryptic Peptides and Amino Acid Sequence of the Amino-Terminal Region of alpha2-CB3 from Chick Skin

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Authors

SDSaryu N. DixitNorthwestern University JSJerome M. SeyerRutgers, The State University of New Jersey AKAndrew H. KangCornell University

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Implication

Structural analysis reveals 80% sequence conservation of the collagen alpha2-CB3 amino-terminal region in chick skin, indicating evolutionary preservation across species.

Key Points

To isolate chymotryptic peptides and determine the complete amino acid sequence of the amino-terminal segment of the alpha2-CB3 fragment in chick skin collagen.
Fractionated chymotryptic peptides from chick skin collagen alpha2-CB3 using a combination of ion-exchange and molecular sieve chromatography.
Established peptide alignment through isolation and sequencing of overlapping tryptic peptide fragments.
Performed automated Edman degradation to sequence 132 amino acid residues across three consecutive amino-terminal chymotryptic peptides.
Five chymotryptic peptides were isolated that together account for the complete amino acid composition of parent peptide alpha2-CB3.
The 132-residue amino-terminal sequence demonstrated approximately 80% identity with homologous skin collagen segments in rats and calves.
Extensive amino acid substitutions were detected when comparing the sequence to the corresponding alpha1 chain region from the same avian species.

Cite This Study

Dixit et al. (1977) studied this question.

synapsesocial.com/papers/6a8aa5047f5a142c17c154a6 https://doi.org/10.1111/j.1432-1033.1977.tb11309.x

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Implication

Structural analysis reveals 80% sequence conservation of the collagen alpha2-CB3 amino-terminal region in chick skin, indicating evolutionary preservation across species.

Covalent Structure of Collagen: Isolation of Chymotryptic Peptides and Amino Acid Sequence of the Amino-Terminal Region of alpha2-CB3 from Chick Skin

Discussion

Member takes

Implication

Key Points

Cite This Study

Covalent Structure of Collagen: Isolation of Chymotryptic Peptides and Amino Acid Sequence of the Amino-Terminal Region of alpha2-CB3 from Chick Skin

Discussion

Member takes

Implication

Key Points

Cite This Study

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