Review highlights metabolic reprogramming strategies for complex polyketides in Yarrowia lipolytica, indicating pathways to an industrially viable biomanufacturing platform.
Key Points
To evaluate current limitations and metabolic engineering strategies for developing Yarrowia lipolytica into a programmable, industrial platform for complex polyketide biosynthesis.
Reviewed cellular and metabolic pathways involved in precursor supply, cofactor balance, and polyketide assembly in Yarrowia lipolytica.
Critically evaluated synthetic biology tools and pathway engineering strategies used to overcome host biosynthetic bottlenecks.
Identified key metabolic constraints limiting precursor flux and heterologous enzyme efficiency in complex polyketide pathways.
Established engineering frameworks to optimize lipid metabolism, cellular energy balance, and yield for sustainable industrial-scale production.