Dynamic Interrelation of Cellular Ingredients Relevant to the Biosynthesis of Anthocyanin During Tissue Culture of Carrot Aggregen

In a strain of carrot aggregen*** derived by tissue culture from cambial tissue of a red, lycopene containing carrot called “Kintoki”. anthocyanin was synthesized de novo, in parallel with a simultaneous production of cinnamic acid derivatives, especially caffeic acid. On the contrary, the contents of free amino acids, malate and carotenoids were decreased down to a remarkable degree. In this case, a minor amount of auxine (IAA or 2, 4-D) plays an essential role in the production of these primary as well as secondary metabolites. In these compounds, a significant difference exsists between the aggregen and genuine root in their quantity, but not in their quality, except for the production of anthocyanin. The de novo or preponderant synthesis of phenolic compound observed in this experiment must be reduced, after all, to the modification of primary metabolic pattern prevailing in the aggregen. This was discussed in relation to the biogenesis of phenolic compounds.