Key result
Recombinant R6 antibody expressed via a Sindbis virus vector completely protected mice from paralysis and death due to Sindbis virus infection and cleared infectious virus from the brains of scid mice.
Population
BHK-21 cells, Balb/cJ mice infected with neuroadapted Sindbis virus, and immunodeficient scid mice…
Comparison
Recombinant R6 IgG produced via Sindbis virus… vs R6 purified from ascites fluid
Design
Preclinical
Authors
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May enable preclinical antiviral antibody platforms; leaves open human translation and safety.
The Sindbis virus vector system is a viable and effective tool for the production of functional, biologically active recombinant antiviral antibodies.
Liang et al. (1997) studied Sindbis virus infection. Recombinant R6 IgG (anti-E2 monoclonal antibody) vs. R6 purified from ascites fluid was evaluated on Protection from paralysis and death and clearance of infectious virus. Recombinant R6 antibody expressed via a Sindbis virus vector completely protected mice from paralysis and death due to Sindbis virus infection and cleared infectious virus from the brains of scid mice.
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